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antibodies anti rat alexafluor 488  (Jackson Immuno)


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    Structured Review

    Jackson Immuno antibodies anti rat alexafluor 488
    Antibodies Anti Rat Alexafluor 488, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 95/100, based on 92 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/donkey+anti+rat+alexa+fluor+488/Alexa+Fluor+488+AffiniPure+F(ab')%E2%82%82+Fragment+Donkey+Anti-Rat+IgG/pm41858055-270-12-19
    Average 95 stars, based on 92 article reviews
    antibodies anti rat alexafluor 488 - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    other:

    Article Title: E-cadherin endocytosis promotes non-canonical EGFR:STAT signalling to induce cell death and inhibit heterochromatinisation
    Article Snippet: Donkey anti-rat Alexa Fluor 488 (1:300) , Jackson ImmunoResearch , Cat #712-545-153.

    Immunohistochemistry:

    Article Title: Comprehensive analysis of spinal cord inflammatory factors in HIV Tat-induced neuropathy in mice.
    Article Snippet: Despite advancements in HIV treatment, HIV neuropathy, caused by nerve damage following HIV infection, continues to be a major cause of morbidity.. Inflammatory responses both in the central and peripheral nervous systems have been implicated as contributing mechanisms, but the role of the HIV protein Trans-Activator of Transcription (Tat) in HIV neuropathy is not well understood.. In this study, we report the development of neuropathy-like behavioral changes in doxycycline-inducible HIV Tat transgenic (iTat) mice using comprehensive behavioral assessments.

    Blocking Assay:

    Article Title: Cortical hyperexcitability drives dying forward amyotrophic lateral sclerosis symptoms and pathology in mice.
    Article Snippet: Sections were then incubated overnight in primary antibodies; rat antiCOUP-TF-interacting protein 2 (CTIP2, 1:500, ab18465, Abcam), rabbit anti-phospho-c-Fos (1:200, 25348, Cell Signalling) and chicken anti-red fluorescent protein (RFP, 1:1000; 600–901–379S, Rockland). .. Following washes and blocking (described above), tissue was incubated in secondary antibodies; donkey anti-rabbit Alexa-Fluor 647 (1:400, 711–545–152, Jackson ImmunoResearch), donkey anti-rat Alexa-Fluor 488 (1:400, 712–605–153, Jackson ImmunoResearch) and donkey antichicken Alexa-Fluor 594 (1:400; 703–585–155, Jackson ImmunoResearch) as described above. ..

    Incubation:

    Article Title: Cortical hyperexcitability drives dying forward amyotrophic lateral sclerosis symptoms and pathology in mice.
    Article Snippet: Sections were then incubated overnight in primary antibodies; rat antiCOUP-TF-interacting protein 2 (CTIP2, 1:500, ab18465, Abcam), rabbit anti-phospho-c-Fos (1:200, 25348, Cell Signalling) and chicken anti-red fluorescent protein (RFP, 1:1000; 600–901–379S, Rockland). .. Following washes and blocking (described above), tissue was incubated in secondary antibodies; donkey anti-rabbit Alexa-Fluor 647 (1:400, 711–545–152, Jackson ImmunoResearch), donkey anti-rat Alexa-Fluor 488 (1:400, 712–605–153, Jackson ImmunoResearch) and donkey antichicken Alexa-Fluor 594 (1:400; 703–585–155, Jackson ImmunoResearch) as described above. ..

    Article Title: MicroRNA210 regulated brain pericyte dysfunction exacerbates hypoxic-ischemic brain injury in neonatal mice
    Article Snippet: Slices were first blocked with 5% donkey serum (017-000-121; Jackson ImmunoResearch, West Grove, PA, USA) containing 0.3% Triton X-100 (X100, Sigma-Aldrich) at room temperature for 1 h, then incubated at 4 °C overnight with primary antibodies: goat anti-CD13 antibody (AF2335; 1:500, R&D, Minneapolis, MN, USA) and rat anti-CD31 antibody (550374; 1:500, BD, San Jose, CA, USA). .. After washing, sections were incubated at room temperature for 1 h with secondary antibodies: donkey anti-goat Alexa Fluor 594 (A-11058; 1:500, Invitrogen, Carlsbad, CA, USA) and donkey anti-rat Alexa Fluor 488 (712-545-150; 1:500, Jackson ImmunoResearch). .. After washing, tissue slices were mounted with fluorescent mounting media (H-2000, Vector Lab) and scanned with a Zeiss LSM 710 confocal microscope (Zeiss) (20x objective, z-stack, around 15-micron projection).

    Article Title: ULK1 Knockout Exacerbates Ischemia-Induced Microglial Dysfunction via TRAF6/NF-κB Signaling Pathway.
    Article Snippet: Activated microglia rapidly migrate to the infarct site, mediate neuroinflammation, and phagocytose cell debris during the acute stage of ischemic stroke; however, the underlying mechanisms remain unclear.. In this study, we utilized a cortical photothrombotic ischemic model and found that unc-51-like autophagy activating kinase 1 (ULK1) knockout mice exhibited increased pro-inflammatory microglia, along with upregulated levels of pro-inflammatory mediators.. Further studies revealed that ULK1 deletion impaired the phagocytosis of myelin debris by microglia, thereby exacerbating myelin accumulation in the infarct zone and increasing pro-inflammatory phagocytic microglia.



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